| Item type |
Journal Article(1) |
| 公開日 |
2020-09-04 |
| タイトル |
|
|
タイトル |
Chicken stem cell factor enhances primordial germ cell proliferation cooperatively with fibroblast growth factor 2 |
| 作成者(その他言語) |
MIYAHARA, Daichi
OISHI, Isao
MAKINO, Ryuichi
KURUMISAWA, Nozomi
NAKAYA, Ryuma
ONO, Tamao
KAGAMI, Hiroshi
TAGAMI, Takahiro
|
| 公開者(その他言語) |
|
|
|
姓名 |
THE, SOCIETY FOR REPRODUCTION AND DEVELOPMENT |
| 書誌情報 |
Journal of Reproduction and Development
巻 62,
号 2,
p. 143-149,
発行日 2016
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Chicken |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Germline chimera |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Primordial germ cells (PGCs) |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Stem cell factor (SCF) |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
metadata only access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
An in vitro culture system of chicken primordial germ cells (PGCs) has been recently developed, but the growth factor involved in the proliferation of PGCs is largely unknown. In the present study, we investigated the growth effects of chicken stem cell factor (chSCF) on the in vitro proliferation of chicken PGCs. We established two feeder cell lines (buffalo rat liver cells; BRL cells) that stably express the putative secreted form of chSCF (chSCF1-BRL) and membrane bound form of chSCF (chSCF2-BRL). Cultured PGC lines were incubated on chSCF1 or chSCF2-BRL feeder cells with fibroblast growth factor 2 (FGF2), and growth effects of each chSCF isoform were investigated. The in vitro proliferation rate of the PGCs cultured on chSCF2-BRL at 20 days of culture was more than threefold higher than those cultured on chSCF1-BRL cells and more than fivefold higher than those cultured on normal BRL cells. Thus, use of chSCF2-BRL feeder layer was effective for in vitro proliferation of chicken PGCs. However, the acceleration of PGC proliferation on chSCF2-BRL was not observed without FGF2, suggesting that chSCF2 would act as a proliferation co-factor of FGF2. We transferred the PGCs cultured on chSCF2-BRL cells to recipient embryos, generated germline chimeric chickens and assessed the germline competency of cultured PGCs by progeny test. Donor-derived progenies were obtained, and the frequency of germline transmission was 3.39%. The results of this study demonstrate that chSCF2 induces hyperproliferation of chicken PGCs retaining germline competency in vitro in cooperation with FGF2. |
| 日付 |
|
|
日付 |
2020-02-28 |
|
日付タイプ |
Created |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Article |
| その他の資源識別子 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Journal of Reproduction and Development.62(2):143-149(2016) |
| 資源識別子URI |
|
|
識別子 |
http://hdl.handle.net/10091/00021882 |
|
識別子タイプ |
HDL |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0916-8818 |
| 処理レコードID(総合目録DB) |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10936678 |
| PubMed番号 |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
https://www.ncbi.nlm.nih.gov/pubmed/26727404 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1262/jrd.2015-128 |
| 権利 |
|
|
権利情報 |
This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license. https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利 |
|
|
権利情報 |
This article is licensed under a Creative Commons [Attribution-NonCommercial-NoDerivatives 4.0 International] license. https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
https://soar-ir.repo.nii.ac.jp/?action=repository_action_common_download&item_id=21125&item_no=1&attribute_id=65&file_no=1
