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Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts
https://shinshu.repo.nii.ac.jp/records/44395
https://shinshu.repo.nii.ac.jp/records/44395df120c37-713c-4a69-b72b-ccabb6a497b9
名前 / ファイル | ライセンス | アクション |
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https://soar-ir.repo.nii.ac.jp/?action=repository_action_common_download&item_id=20696&item_no=1&attribute_id=65&file_no=1
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Item type | Journal Article(1) | |||||
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公開日 | 2020-09-04 | |||||
タイトル | ||||||
タイトル | Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts | |||||
作成者(その他言語) |
Takaya, Tomohide
× Takaya, Tomohide× Nihashi, Yuma× Kojima, Shotaro× Ono, Tamao× Kagami, Hiroshi |
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公開者(その他言語) | ||||||
姓名 | WILEY | |||||
書誌情報 |
ANIMAL SCIENCE JOURNAL 巻 88, 号 11, p. 1880-1885, 発行日 2017-11 |
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言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cell fusion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hybrid cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | myotube formation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | skeletal muscle myoblast | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Cell-cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB-GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB-DsRed. mMB-GFP and chMB-DsRed were cocultured and induced to differentiate. After 24h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP(+)/DsRed(+) hybrid myotubes were able to survive and grew to hyper-multinucleated mature form. We also found that undifferentiated mMB-GFP efficiently fuse to the chMB-DsRed-derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast-based fusogenic technique will open up an alternative direction to create novel hybrid products. | |||||
日付 | ||||||
日付 | 2019-05-08 | |||||
日付タイプ | Created | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
その他の資源識別子 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ANIMAL SCIENCE JOURNAL. 88(11):1880-1885 (2017) | |||||
資源識別子URI | ||||||
識別子 | http://hdl.handle.net/10091/00021454 | |||||
識別子タイプ | HDL | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1344-3941 | |||||
処理レコードID(総合目録DB) | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11677214 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=28782148 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1111/asj.12884 | |||||
権利 | ||||||
権利情報 | © 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |||||
権利 | ||||||
権利情報 | © 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |