{"created":"2023-05-15T16:12:15.167822+00:00","id":42681,"links":{},"metadata":{"_buckets":{"deposit":"c4ba4954-a05c-488c-a17b-fce9ba6b3313"},"_deposit":{"created_by":25,"id":"42681","owners":[25],"pid":{"revision_id":0,"type":"depid","value":"42681"},"status":"published"},"_oai":{"id":"oai:shinshu.repo.nii.ac.jp:00042681","sets":[]},"author_link":["166594","166593","166590","166592","166589","166591"],"item_20002_alternative_title_1":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"胎盤抽出物がヒト骨肉腫細胞株Saos-2の細胞増殖、I型コラーゲン産生およびALP分泌に及ぼす効果"}]},"item_20002_biblio_info_27":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"160","bibliographicPageStart":"157","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Journal of Hard Tissue Biology"}]}]},"item_20002_creator_3":{"attribute_name":"作成者(その他言語)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"IMAMURA, YASUHIRO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HONDA, YOSHITOMO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MASUNO, KAZUYA"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NAKAMURA, HIROE"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"WANG, PAO-LI"}],"nameIdentifiers":[{}]}]},"item_20002_date_19":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2018-03-06","subitem_date_issued_type":"Created"}]},"item_20002_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Porcine placenta extract (P-placenta) is widely applied in medicine and cosmetics. However, few studies have examined the effect of the extract on the cellular behavior of the osteoblastic cell line Saos-2. Here, we demonstrated that P-placenta enhances the proliferation, collagen type I production, and alkaline phosphatase (ALP) secretion of Saos-2 in vitro. Proliferation of Saos-2 was assessed by MTT and DNA synthesisassays. Type I collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. The cells were treated with/without 20, 200 and 2000 g/ml of P-placenta for 24 h. We found that 200 g/ml P-placenta significantly induced the proliferation of Saos-2 and enhanced type I collagen production and ALP secretion. The results indicate that P-placenta controls the cellular behavior of osteoblasts,resulting in the secretion of early bone-related biomarkers.","subitem_description_type":"Other"}]},"item_20002_description_21":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_20002_full_name_16":{"attribute_name":"公開者(その他言語)","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"Society, for Hard Tissue Regenerative Biology (硬組織再生生物学会)"}]}]},"item_20002_identifier_23":{"attribute_name":"資源識別子URI","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://id.nii.ac.jp/1070/00002698/"}]},"item_20002_relation_28":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版)"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.2485/jhtb.26.157"}]}]},"item_20002_relation_32":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.2485/jhtb.26.157","subitem_relation_type_select":"DOI"}}]},"item_20002_relation_59":{"attribute_name":"NII論文ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://ci.nii.ac.jp/naid/40021177219","subitem_relation_type_select":"NAID"}}]},"item_20002_relation_60":{"attribute_name":"医中誌ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://search.jamas.or.jp/link/ui/2018007458","subitem_relation_type_select":"ICHUSHI"}}]},"item_20002_rights_50":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017 by The Hard Tissue Biology Network Association(JHTBNet)"}]},"item_20002_source_id_25":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1341-7649","subitem_source_identifier_type":"ISSN"}]},"item_20002_source_id_26":{"attribute_name":"処理レコードID(総合目録DB)","attribute_value_mlt":[{"subitem_source_identifier":"AA11074332","subitem_source_identifier_type":"NCID"}]},"item_20002_version_type_51":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"url":{"url":"https://mdu.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2707&item_no=1&attribute_id=22&file_no=1"}}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"骨肉腫","subitem_subject_scheme":"Other"},{"subitem_subject":"胎盤抽出物","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞増殖","subitem_subject_scheme":"Other"},{"subitem_subject":"腫瘍細胞系","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒト","subitem_subject_scheme":"Other"},{"subitem_subject":"ブタ","subitem_subject_scheme":"Other"},{"subitem_subject":"動物","subitem_subject_scheme":"Other"},{"subitem_subject":"ALP","subitem_subject_scheme":"Other"},{"subitem_subject":"Collagen","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoblast","subitem_subject_scheme":"Other"},{"subitem_subject":"Placenta","subitem_subject_scheme":"Other"},{"subitem_subject":"Saos-2","subitem_subject_scheme":"Other"},{"subitem_subject":"Alkaline Phosphatase","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_scheme":"Other"},{"subitem_subject":"Tetrazolium Salts","subitem_subject_scheme":"Other"},{"subitem_subject":"Thiazoles","subitem_subject_scheme":"Other"},{"subitem_subject":"Collagen Type I","subitem_subject_scheme":"Other"},{"subitem_subject":"Thiazolyl Blue","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2","subitem_title_language":"en"}]},"item_type_id":"20002","owner":"25","path":["2527","2625"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-04-01"},"publish_date":"2021-04-01","publish_status":"0","recid":"42681","relation_version_is_last":true,"title":["Effects of Placental Extract on Cell Proliferation, Type I Collagen Production, and ALP Secretion in Human Osteosarcoma Cell Line Saos-2"],"weko_creator_id":"25","weko_shared_id":-1},"updated":"2023-05-15T17:52:49.928656+00:00"}