{"created":"2023-05-15T16:11:30.393356+00:00","id":41886,"links":{},"metadata":{"_buckets":{"deposit":"7a69c8a9-817e-4a39-adcd-e4a5bc9f3a69"},"_deposit":{"created_by":25,"id":"41886","owners":[25],"pid":{"revision_id":0,"type":"depid","value":"41886"},"status":"published"},"_oai":{"id":"oai:shinshu.repo.nii.ac.jp:00041886","sets":[]},"author_link":["164205","164207","164208","164206"],"item_20002_biblio_info_27":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-02-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"Article ID 784019","bibliographicVolumeNumber":"2014","bibliographic_titles":[{"bibliographic_title":"ISRN pharmacology"}]}]},"item_20002_creator_3":{"attribute_name":"作成者(その他言語)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kitamura, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Urano, Hiroko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ara, Toshiaki"}],"nameIdentifiers":[{}]}]},"item_20002_date_19":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2017-05-18","subitem_date_issued_type":"Created"}]},"item_20002_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Periodontal disease is accompanied by inflammation of the gingiva and destruction of periodontal tissues, leading to alveolar bone loss in severe clinical cases. The chemical mediator prostaglandin E2 (PGE2) and cytokines such as interleukin- (IL-)6 and IL-8 have been known to play important roles in inflammatory responses and tissue degradation. In the present study, we investigated the effects of a kampo medicine, kakkonto (TJ-1), on the production of prostaglandin E2 (PGE2), IL-6, and IL-8 by human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) from Porphyromonas gingivalis. Kakkonto concentration dependently suppressed LPS-induced PGE2 production but did not alter basal PGE2 levels. In contrast, kakkonto significantly increased LPSinduced IL-6 and IL-8 production. Kakkonto decreased cyclooxygenase- (COX-)1 activity to approximately 70% at 1mg/mL but did not affect COX-2 activity. Kakkonto did not affect cytoplasmic phospholipase A2 (cPLA2), annexin1, or LPS-induced COX-2 expression. Kakkonto suppressed LPS-induced extracellular signal-regulated kinase (ERK) phosphorylation, which is known to lead to ERK activation and cPLA2 phosphorylation. These results suggest that kakkonto decreased PGE2 production by inhibition of ERK phosphorylation which leads to inhibition of cPLA2 phosphorylation and its activation. Therefore, kakkonto may be useful to improve gingival inflammation in periodontal disease.","subitem_description_type":"Other"}]},"item_20002_description_21":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_20002_full_name_16":{"attribute_name":"公開者(その他言語)","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"Hindawi, Publishing Corporation"}]}]},"item_20002_identifier_23":{"attribute_name":"資源識別子URI","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://id.nii.ac.jp/1070/00002660/"}]},"item_20002_relation_28":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版)"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.hindawi.com/journals/isrn/2014/784019/"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945151/"}]}]},"item_20002_relation_31":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/24693448","subitem_relation_type_select":"PMID"}}]},"item_20002_relation_32":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1155/2014/784019","subitem_relation_type_select":"DOI"}}]},"item_20002_rights_50":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2014 Hiroyuki Kitamura et al. This is an open access article distributed under the Creative Commons Attribution License(Attribution 3.0 Unported (CC BY 3.0)  ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited."},{"subitem_rights":"CC BY"}]},"item_20002_source_id_25":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2090-5173","subitem_source_identifier_type":"ISSN"}]},"item_20002_version_type_51":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"url":{"url":"https://mdu.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2669&item_no=1&attribute_id=22&file_no=1"}}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Preventive Effects of a Kampo Medicine, Kakkonto, on Inflammatory Responses via the Suppression of Extracellular Signal-Regulated Kinase Phosphorylation in Lipopolysaccharide-Treated Human Gingival Fibroblasts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Preventive Effects of a Kampo Medicine, Kakkonto, on Inflammatory Responses via the Suppression of Extracellular Signal-Regulated Kinase Phosphorylation in Lipopolysaccharide-Treated Human Gingival Fibroblasts","subitem_title_language":"en"}]},"item_type_id":"20002","owner":"25","path":["2513","2655","2772","2775"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-04-01"},"publish_date":"2021-04-01","publish_status":"0","recid":"41886","relation_version_is_last":true,"title":["Preventive Effects of a Kampo Medicine, Kakkonto, on Inflammatory Responses via the Suppression of Extracellular Signal-Regulated Kinase Phosphorylation in Lipopolysaccharide-Treated Human Gingival Fibroblasts"],"weko_creator_id":"25","weko_shared_id":-1},"updated":"2023-05-15T17:53:05.517787+00:00"}