{"created":"2023-05-15T16:10:26.733255+00:00","id":40737,"links":{},"metadata":{"_buckets":{"deposit":"bfa14594-be51-4ff9-a805-226d318654d8"},"_deposit":{"created_by":25,"id":"40737","owners":[25],"pid":{"revision_id":0,"type":"depid","value":"40737"},"status":"published"},"_oai":{"id":"oai:shinshu.repo.nii.ac.jp:00040737","sets":[]},"author_link":["160614","160622","160616","160619","160621","160618","160613","160615","160617","160620"],"item_20003_alternative_title_1":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"歯科矯正学的メカニカルストレスによるマウス歯周組織改造における細胞動態"}]},"item_20003_biblio_info_27":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-08-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"694","bibliographicPageStart":"689","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"International Journal of Medical Sciences"}]}]},"item_20003_creator_3":{"attribute_name":"作成者(その他言語)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kaneko, Keiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuda, Sakae"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muraoka, Rina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakano, Keisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwasaki, Takami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomida, Mihoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsujigiwa, Hidetsugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagatsuka, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"金子, 圭子"}],"nameIdentifiers":[{}]}]},"item_20003_date_19":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2016-06-02","subitem_date_issued_type":"Created"}]},"item_20003_date_granted_56":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-02-05"}]},"item_20003_degree_grantor_58":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"松本歯科大学"}],"subitem_degreegrantor_identifier":[{}]}]},"item_20003_degree_name_57":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_20003_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"The purpose of the study was to determine the cell dynamics in periodontal ligament in response to mechanical stress during orthodontic movement. Following Waldo’s method, a square sheet of rubber dam was inserted in between the first and second maxillary molars in 10 ddY mice leaving the stress load for 3 hours. After 3 days and at 1 week, cell count on pressure and tension sides of the periodontal ligament was determined. Furthermore, the type of cell present after mechanical stress was identified using GFP bone marrow transplantation mouse model. Immunohistochemistry was carried out at 0 min (immediately after mechanical stress), 24 hours, 1 week, 2 weeks and 6 months. Temporal changes in the expression of GFP-positive bone marrow derived cells were examined. Moreover, double immunofluorescent staining was performed to determine the type of cell in the periodontal ligament. Cell count on the tension side tremendously increased 3 days after mechanical stress. At 1 week, spindle and round cell count increased compared to the control group. These changes were observed on both tension and pressure sides. Cell count on pressure side at 3 days (22.11+/-13.98) and at 1 week (33.23+/-11.39) was higher compared to the control group (15.26+/-8.29). On the tension side, there was a significantly increased at 3 days (35.46+/-11.85), but decreased at 1 week (29.23+/-13.89) although it is still higher compared to the control group (AD+/-SD: 10.37+/-8.69). Using GFP bone marrow transplantation mouse model, GFP positive cell count increased gradually over time in 6 months. GFP positive cells were also positive to CD31, CD68 and Runx2 suggesting that fibroblasts differentiated into osteoclasts and tissue macrophages. In conclusion, mechanical stress during orthodontic movement promoted the increase in the number of cells in the periodontal ligament on both tension and pressure sides. The increase in the number of cells in the periodontal ligament is believed to be due to the migration and cell division of undifferentiated mesenchymal cells.","subitem_description_type":"Other"},{"subitem_description":"2015","subitem_description_type":"Other"}]},"item_20003_description_21":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_20003_description_22":{"attribute_name":"その他の資源識別子","attribute_value_mlt":[{"subitem_description":"甲第186号","subitem_description_type":"Other"}]},"item_20003_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第186号"}]},"item_20003_identifier_23":{"attribute_name":"資源識別子URI","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://id.nii.ac.jp/1070/00002507/"}]},"item_20003_relation_28":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"雑誌掲載論文(電子版)"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.medsci.org/v12p0689.htm"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571545/"}]}]},"item_20003_relation_31":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:pmid/26392805","subitem_relation_type_select":"PMID"}}]},"item_20003_relation_32":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.7150/ijms.12883","subitem_relation_type_select":"DOI"}}]},"item_20003_rights_50":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2015 Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License.(https://creativecommons.org/licenses/by-nc/4.0/)"},{"subitem_rights":"CC BY-NC-ND"}]},"item_20003_source_id_25":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1449-1907","subitem_source_identifier_type":"ISSN"}]},"item_20003_source_id_26":{"attribute_name":"処理レコードID(総合目録DB)","attribute_value_mlt":[{"subitem_source_identifier":"AA1197928X","subitem_source_identifier_type":"NCID"}]},"item_20003_version_type_51":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"url":{"url":"https://mdu.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2516&item_no=1&attribute_id=20&file_no=2"}},{"url":{"url":"https://mdu.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2516&item_no=1&attribute_id=20&file_no=1"}}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Periodontal Ligament","subitem_subject_scheme":"Other"},{"subitem_subject":"Orthodontic Mechanical Stress","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell movement","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell differentiation","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunohistochemistry","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Histological Evaluation of Periodontal Ligament in Response to Orthodontic Mechanical Stress in Mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Histological Evaluation of Periodontal Ligament in Response to Orthodontic Mechanical Stress in Mice","subitem_title_language":"en"}]},"item_type_id":"20003","owner":"25","path":["2612"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-10"},"publish_date":"2022-11-10","publish_status":"0","recid":"40737","relation_version_is_last":true,"title":["Histological Evaluation of Periodontal Ligament in Response to Orthodontic Mechanical Stress in Mice"],"weko_creator_id":"25","weko_shared_id":25},"updated":"2023-05-15T18:07:05.412030+00:00"}