{"created":"2023-05-15T15:55:10.090269+00:00","id":23621,"links":{},"metadata":{"_buckets":{"deposit":"6e38df83-3b5c-4216-92a9-c8708ce6a6be"},"_deposit":{"created_by":1,"id":"23621","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"23621"},"status":"published"},"_oai":{"id":"oai:shinshu.repo.nii.ac.jp:00023621","sets":[]},"author_link":["95670","95661","95665","95669","95663","95668","95667","95662","95666","95664"],"item_20003_alternative_title_1":{"attribute_name":"その他(別言語等)のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Porphyromonas gingivalisのプロリルトリペプチジルペプチダーゼの産生，単離および性状"}]},"item_20003_creator_3":{"attribute_name":"作成者(その他言語)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Matsuo, Kazuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kiso, Akiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Yukinaga"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hasegawa, Hiromasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujimura, Setsuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松尾, 和子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"木曽, 有紀子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"柴田, 幸永"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長谷川, 博雅"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"藤村, 節夫"}],"nameIdentifiers":[{}]}]},"item_20003_date_19":{"attribute_name":"日付","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-07-06","subitem_date_issued_type":"Created"}]},"item_20003_date_granted_56":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-02-05"}]},"item_20003_degree_grantor_58":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"松本歯科大学"}],"subitem_degreegrantor_identifier":[{}]}]},"item_20003_degree_name_57":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_20003_description_14":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Formation, cellular locations, isolation and enzymatic propertiesof PTP of Porphyromonas gingivalis, an anaerobic periodontalpathogen were investigated.Almost all activities of this enzyme were detected in the crudeextract of the cell, but the other bacterial fractions such as culturefluids, envelopes and vesicles were not found to contain PTP.PTP was purified from the crude extract prepared by sonicationand centrifugation through five steps including concentration, ionexchange chromatography, gel filtration, hydrophobic interactionchromatography and isoelectric focusing to homogeneity.The enzyme was a serine enzyme since it was inhibited stronglyby Pefabloc SC, diisopropyl fluorophosphate and3,4-dichloroisocoumarin. It hydrolyzed H-Ala-Ala-Pro-pNA andH-Ala-Phe-Pro-pNA. The molecular mass was determined as 45kDa and isoelectric point was 5.7.Optimum pH was moderately broad, and maximum activity wasobserved in the range of pH 7.0 to 9.0.The residual activity after heating at 50℃ for 5 min was 29%,but heating at 60℃ resulted in complete loss of the activity.","subitem_description_type":"Other"},{"subitem_description":"2014","subitem_description_type":"Other"}]},"item_20003_description_21":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_20003_description_22":{"attribute_name":"その他の資源識別子","attribute_value_mlt":[{"subitem_description":"甲第167号","subitem_description_type":"Other"}]},"item_20003_dissertation_number_55":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第167号"}]},"item_20003_identifier_23":{"attribute_name":"資源識別子URI","attribute_value_mlt":[{"subitem_identifier_type":"URI","subitem_identifier_uri":"http://id.nii.ac.jp/1070/00002403/"}]},"item_20003_relation_28":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://mdu.repo.nii.ac.jp/?action=pages_view_main&active_action=repository_view_main_item_detail&item_id=2266&item_no=1&page_id=13&block_id=17"}]}]},"item_20003_relation_30":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"投稿雑誌：Matsuo Kazuko, Kiso Akiko, Shibata Yukinaga, Hasegawa Hiromasa, Fujimura Setsuo. Formation, isolation and characterization of a prolyl tripeptidyl peptidase of Porphyromonas gingivalis. 松本歯学(0385-1613)40巻1号 Page1-12(2014.06). © 2016 松本歯科大学学会"}]}]},"item_20003_version_type_51":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"url":{"url":"https://mdu.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2415&item_no=1&attribute_id=20&file_no=1"}},{"url":{"url":"https://mdu.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2415&item_no=1&attribute_id=20&file_no=2"}}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"enzyme","subitem_subject_scheme":"Other"},{"subitem_subject":"prolyl tripeptidase","subitem_subject_scheme":"Other"},{"subitem_subject":"Porphyromonas gingivalis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Formation, isolation and characterization of a prolyl tripeptidyl peptidase of Porphyromonas gingivalis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Formation, isolation and characterization of a prolyl tripeptidyl peptidase of Porphyromonas gingivalis","subitem_title_language":"en"}]},"item_type_id":"20003","owner":"1","path":["2589"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-11-10"},"publish_date":"2022-11-10","publish_status":"0","recid":"23621","relation_version_is_last":true,"title":["Formation, isolation and characterization of a prolyl tripeptidyl peptidase of Porphyromonas gingivalis"],"weko_creator_id":"1","weko_shared_id":1},"updated":"2023-05-15T18:13:54.210755+00:00"}