@article{oai:shinshu.repo.nii.ac.jp:00002080,
 author = {田坂, 裕子 and 洞沢, 功子 and 高橋, 重雄 and 上松, 隆司 and 長谷川, 貴史},
 issue = {1},
 journal = {松本歯学},
 month = {Apr},
 note = {Toxic effects of four metal cations released generally from dental casting alloys were evaluated by corrosion test and in vitro cytotoxicity. Corrosion tests were conducted on four pure metal plates (Cu, Zn, Ag, Pd), and immersed in human resting saliva for 3 weeks at 37℃. Quantitative analysis of the metal cations released into the saliva was performed by the I.C.P. (inductively coupled plasma emission spectrometry) method. In vitro cytotoxicity was examined using L-929 cell lines. The cell line to be tested was adjusted to 2×10^4cells/ml in growth medium containing cupper and zinc ions at various concentrations. Cytotoxicity was determined to have occurred if a 50% inhibitory concentration was found when compared to controls (IC_<50>). The results obtained were as follows. 1. The amount of cupper ion released into human saliva was 274μg/cm^2. This was equal with the amount of nickel ions released from some Ni-Cr alloys. 2. The amount of zinc ions released was 14μg/cm^2. The release was restrained by white matter deposited on the surface of the metal plate, 3 days later. 3. Silver and Palladium were excellent for the inhibition of corrosion to human saliva. 4. The IC_<50> of the copper ion was 1.28ppm (20.2μM) and of the zinc ion was 10.6ppm (159μM) in the case that the surface area of metal restration was 1cm^2, and the quantity of saliva was 1L/day. 5. The amount of copper ions to the IC_<50> reached immediately after metal restration was set, and all cells died in a day. 6. The amount of zinc ions to the IC_<50> reached in 2～3 days. However, no increase of concentration occurred after that, and did not reach the concentration at which all cells died., application/pdf},
 pages = {64--69},
 title = {歯科用金属の安全性試験 : 金属イオンの細胞障害性について},
 volume = {20},
 year = {1994}
}