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A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
https://shinshu.repo.nii.ac.jp/records/27025
https://shinshu.repo.nii.ac.jp/records/2702508138d9e-ebed-4ca3-a20b-78dab7580944
名前 / ファイル | ライセンス | アクション |
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https://soar-ir.repo.nii.ac.jp/?action=repository_action_common_download&item_id=3679&item_no=1&attribute_id=65&file_no=1
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Item type | Journal Article(1) | |||||
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公開日 | 2020-09-01 | |||||
タイトル | ||||||
タイトル | A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min | |||||
作成者(その他言語) |
Uehara, Masayuki
× Uehara, Masayuki× Matsuda, Kazuyuki× Sugano, Mitsutoshi× Honda, Takayuki |
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公開者(その他言語) | ||||||
姓名 | JAPAN, SOC VET SCI | |||||
書誌情報 |
JOURNAL OF VETERINARY MEDICAL SCIENCE 巻 76, 号 3, p. 477-480, 発行日 2014-03 |
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言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bovine respiratory syncytial virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | domestic animal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | droplet-real-time PCR | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | infectious disease | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus. | |||||
日付 | ||||||
日付 | 2015-09-24 | |||||
日付タイプ | Created | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
その他の資源識別子 | ||||||
内容記述タイプ | Other | |||||
内容記述 | JOURNAL OF VETERINARY MEDICAL SCIENCE. 76(3):477-480 (2014) | |||||
資源識別子URI | ||||||
識別子 | http://hdl.handle.net/10091/17830 | |||||
識別子タイプ | HDL | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-7250 | |||||
処理レコードID(総合目録DB) | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10796138 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=24285011 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.1292/jvms.13-0357 | |||||
権利 | ||||||
権利情報 | Copyright© 2014 The Japanese Society of Veterinary Science | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |