| Item type |
Journal Article(1) |
| 公開日 |
2020-09-01 |
| タイトル |
|
|
タイトル |
Proteomics-based safety evaluation of multi-walled carbon nanotubes |
| 作成者(その他言語) |
Haniu, Hisao
Matsuda, Yoshikazu
Takeuchi, Kenji
Kim, Yoong Ahm
Hayashi, Takuya
Endo, Morinobu
|
| 公開者(その他言語) |
|
|
|
姓名 |
ACADEMIC, PRESS INC ELSEVIER SCIENCE |
| 書誌情報 |
Applied Pharmacology
巻 242,
号 3,
p. 256-262,
発行日 2010-02-01
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Carbon nanotubes |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Safety evaluation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Proteomics |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Protein expression |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
In vitro |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
metadata only access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This study evaluated the biological responses to multi-walled carbon nanotubes (MWCNTs). Human monoblastic leukemia cells (U937) were exposed to As-grown MWCNTs and MWCNTs that were thermally treated at 1800 degrees C (HTT1800) and 2800 degrees C (HTT2800). Cell proliferation was highly inhibited by As-grown but not HTT2800. However, both As-grown and HTT1800, which include some impurities, were cytotoxic. Proteomics analysis of MWCNT-exposed cells revealed 37 protein spots on 2-dimensional electrophoresis gels that significantly changed (p<0.05) after exposure to HTT1800 with a little iron and 20 spots that changed after exposure to HTT2800. Peptide mass fingerprinting identified 45 proteins that included heat shock protein beta-1, neutral alpha-glucosidase AB, and DNA mismatch repair protein Msh2. These altered proteins play roles in metabolism, biosynthesis, response to stress, and cell differentiation. Although HTT2800 did not inhibit cell proliferation or cause cytotoxicity in vitro, some proteins related to the response to stress were changed. Moreover, DJ-1 protein, which is a biomarker of Parkinson's disease and is related to cancer, was identified after exposure to both MWCNTs. These results show that the cytotoxicity of MWCNTs depends on their impurities, such as iron, while MWCNTs themselves cause some biological responses directly and/or indirectly in vitro. Our proteomics-based approach for detecting biological responses to nanomaterials is a promising new method for detailed safety evaluations. |
| 日付 |
|
|
日付 |
2015-09-18 |
|
日付タイプ |
Created |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Article |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| その他の資源識別子 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Toxicology and Applied Pharmacology. 242(3):256-262 (2010) |
| 資源識別子URI |
|
|
識別子 |
http://hdl.handle.net/10091/10727 |
|
識別子タイプ |
HDL |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0041-008x |
| 処理レコードID(総合目録DB) |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00864435 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=19874835 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.taap.2009.10.015 |
| 権利 |
|
|
権利情報 |
Copyright © 2009 Elsevier Inc. |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
https://soar-ir.repo.nii.ac.jp/?action=repository_action_common_download&item_id=366&item_no=1&attribute_id=65&file_no=1
